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KMID : 0545120110210121236
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 12 p.1236 ~ p.1242
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Screening and Identification of Antimicrobial Compounds from Streptomyces bottropensis Suppressing Rice Bacterial Blight
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Park Sait-Byul
Lee In-Ae
Suh Joo-Won
Kim Jeong-Gu
Lee Choong-Hwan
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Abstract
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Xanthomonas oryzae pv. oryzae (Xoo) is the most devastating pathogen to Oryza sativa and has been shown to cause bacterial blight. Two bioactive compounds showing antimicrobial activities against Xoo strain KACC 10331 were isolated from a Streptomyces bottropensis strain. The ethyl acetate extract was fractionated on a Sephadex LH-20 column, and then purified by preparative HPLC. The purified compounds were identified as bottromycin A2 and dunaimycin D3S by HR/MS and 1H NMR analyses. The MIC value against Xoo and the lowest concentration still capable of suppressing rice bacterial blight were 2 μg/ml and 16 μg/ml for bottromycin A2, and 64 μg/ml and 0.06 μg/ml for dunaimycin D3S, respectively. These two compounds were shown to exert different bioactivities in vitro and in rice leaf explants.
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KEYWORD
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Streptomyces bottropensis, rice bacterial blight, Bottromycin A2, Dunaimycin D3S, Xanthomonas oryzae pv. oryzae, antibacterial activity
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